MATERIALS AND LABORATORY EQUIPMENTS

For the consultation of professionals in the area, we disponibilize in this part the equipment necessary in an embryology laboratory and the material utilized in the treatment with techniques of assisted reproduction.

EQUIPMENTS AND MATERIALS USED IN THE IVF LABORATORY (Clinic and Research Center in Human Reproduction Roger Abdelmassih)

1 - EQUIPMENTS

2 Inverted microscope ( Nikon – Eclipse )
1 Inverted Microscope ( Nikon Diaphot )
2 Phase Contrast ( Hoffman - Modulation Optics Inc., Greenvale, NY )
2 Videocameras ( Hitachi and Fertilase )
1 Monitor with Video ( Panasonic – Omnivision VHS )
1 Nikon Photographic Camera ( FM2 )
2 Tridimensional Micromanipulators ( 3D motor driven coarse control manipulator MM-188; Narishige )
2 Tridimensional Hydraulic Controls ( Joystick Hydraulic Micromanipulator MO – 188; Narishige )
2 Microinjectors ( IM6 – 800ml – Narishige )
2 Heater Plates ( Fryer A 50; Fryer Co. Inc., Huntley, IL )
9 Incubators ( Forma Scientific – 3110 – Forma Scientific, Inc. – Marietta, Ohio – USA)
1 Laminar Flow Hood ( Edge GARD Hood – The Baker Company, inc. )
1 Refrigerator ( Consul – Slim )
1 Freezer ( Consul – Slim )
1 Ambient Filter System ( Coda Tower - < gen X> international – mod. 4 stage Filter System )
1 Centrifuge ( Fanem – Excelsa II model 206 MP )
1 Heater Plate ( Olidef CZ – model V. V. )
1 Isolete ( IVF Hoffman Chamber )
1 Stereomicroscope ( WILD Heerbrugg )
1 Ultra Pure Water Sistern ( Milli-Q Water System – Millipore )
1 Laser System (Fertilase TM – MTM Medical Technologies Montreux – Switzerland )
1 Volumetric Balance ( OHAUS Analytical Plus )
2 Mary’s Bath Devices ( Fanem  - Model 100 )
1 Hybrite ( Vysis )
1 Vortex ( Genie 2 - Scientific Industries )
1 Freeze-Thaw machine ( Planer KRYO 10 – Serie II )
1 Fixation Microscope (Nikon – TMS) )
1 Fluorescence Microscope (Leitz – Aristoplan )
1 Sealer (Barbi)

2 - PLASTIC MATERIAL

Centrifuge tube 13 ml (Falcon ref. 2001)
Conic Costar tube 15 ml ( ref. 3215/3217)
Centrifuge Tube 4ml (Falcon ref. 2003)
Plate with Center 60 mm Falcon ( ref 3037) or Costar (ref 3260)
Plate without Center 60 mm Falcon (ref.3002) or Costar (ref. 3060)
Plate 30 mm (Costar ref. 3035)
Plate 50 mm (Falcon ref. 1006)
Transfer Pipette 3 ml (Falcon ref. 7575 )
Tips 200 ml (Costar ref. 4864)
Pipette 10 ml (Falcon ref. 7551)
Pipette 5 ml (Falcon ref. 7543)
Millipore  0.22µm Filters – Sterivex (ref. SVGS 01015)
Sterile Pasteur Pipettes Sigma (ref 5893)
Non sterile Pasteur Pipettes 5 ¾ (Costar ref. 5005)
Flask 50 ml (Falcon ref. 3013)
Flask 200 ml (Falcon ref. 3024)
4 well plates Nunc (ref. 176740)
Freeze straws IMV 10 (ref. ZA 479)
Microscope glasses 24X24 mm (Number 1 – Inlab)
Microscope 
Laminilas
Glass mark pen (ref. 750 – Glass mark pen)
Sterile 
Transfer Catheter Edwards – Wallace 23 cm ( ref. ME 1816 N )
Malleable Stylet for Catheter 23 cm ( ref. ME 1816 NST )
Stripper ( MID Atlantic Diagnosis Inc.)
Stripper Tips 200µm ( ref. MXL3 - 200 ); 150µm ( ref. MXLE - 150 ); 125µm ( ref. MXL3 - 125 )
Injection Micropipettes COOK ( ref. K - MPIP - 1035 )
Injection Micropipettes HUMAGEN ( ref. 10- MIC )
Holding Micropipettes COOK ( ref. K - HPIP - 1035 )
Holding Micropipettes HUMAGEN ( ref. 10- MPH-120 )
Biopsy Micropipettes COOK ( ref. K - EBP - 4035 )
Biopsy Micropipettes HUMAGEN ( ref. 10 - MBB )
Hatching Micropipettes COOK ( ref. K - AHP - 1035 )
Hatching Micropipettes HUMAGEN ( ref. 10- MAH )

EQUIPMENT PHOTOS

CULTURE MEDIA ( Clinic and Research Center for Human Reproduction Roger Abdelmassih )

Scandinavia IVF science

GAMETE 100 – Ref. no 1004 = Injection medium and hyaluronidase dissolution.

IVF 50 – Ref. no. 2004 = Fertilization, embryo culture and embryo transfer (day 2 and 3)

G1.2 – Ref no. 8010 = Embryo culture and embryo transfer. Embryo culture until the 6-8 cell stage followed by G2.2 medium. Do not use for fertilization.

G2.2 – Ref. no. 8020 = Embryo culture from the 6-8 cell stage until the blastocyst stage and for blastocyst transference.

OVOIL 150 – Ref no. 5002 = To cover all IVF media and in the process of micromanipulation

ICSI 100 – Ref. no. 1100 = Spermatozoon immobilization.

HYASE 1 – Ref. no. 1300 = Granulosa Cell Removal.

FREEZE-KIT 1 – Ref. no. 6120 = Embryo freezing in cleavage stage.

THAW-KIT 1 – Ref. no. 6130 = Embryo thawing in cleavage stage.

FREEZE-KIT 2 – Ref. no. 6320 = Embryo freezing in blastocyst stage.

THAW-KIT 2 – Ref. no. 6330 = Embryo thawing in blastocyst stage.

SPERM RINSE 20 – Ref. no. 7120 = Sperm preparation.

Irvine Scientific

MODIFIED HTF – Ref n. 9963 = Oocyte flushing, aspiration system wash + 5% SSS.

HTF – Ref no. 9963 = Oocyte and embryo culture, sperm wash + 15% SSS

BLASTOCYST MEDIUM – Ref n. = Culture until blastocyst stage + 15% SSS

HYALURONIDASE 80 UI/ML (Sigma Ref. No. H-3757) = Cumulus-Corona complex removal from oocytes with HTF 9962 + 0.5% SSS.

SYNTETHIC SERUM SUBSTITUTE (SSS) – Ref. No. 99193 = Supplement for culture media

HSA - HUMAN SERUM ALBUMIN - Ref.n°. 9988 = Supplement for culture media.

TYRODE’S ACID SOLUTION – Ref. No. 99252 = Assisted Hatching

PVP (Sigma) – Ref. No. P-40 = Sperm immobilization with Modified HTF + 1g PVP + 0.5% SSS.

INJECTION MEDIUM = Modified HTF + 1% SSS.

SIGMA MINERAL OIL – Ref. No. M-8410 = Culture dish preparation and injection previously balanced with HTF